Evaluation of the nociceptive threshold and inflammatory markers in rats after induction of pulmonary emphysema by elastase in the phasic, inflammatory and neuropathic pain models
Avaliação do limiar nociceptivo e marcadores inflamatórios em ratos após indução de enfisema pulmonar por elastase nos modelos de dor fásica, inflamatória e neuropática
Rafaella Rocha Figueiredo; Isabela de Paula Ribeiro Argôlo; Luiz Fernando Xavier; Josie Resende Silva; Marcelo Lourenço Da-Silva
Abstract
BACKGROUND AND OBJECTIVES: Chronic obstructive pulmonary disease (COPD) is characterized by airflow obstruction, although it compromises the lungs, it also produces significant systemic consequences. The objective of this study was to analyze the relationship between pro-inflammatory cytokines and nociceptive threshold in rats with porcine pancreatic elastase-induced COPD.
METHODS: 144 animals were randomly distributed into 3 different models: nociceptive phasic pain at tail-flick test (TF), inflammatory pain in the Freund’s complete adjuvant model (CFA) and neuropathic pain in the sciatic nerve constriction model (CCI). 21 days after tracheal instillation of elastase the COPD was established, and the nociceptive threshold was evaluated at different times.
RESULTS: The animals with COPD in TF had a shorter latency time and higher levels of IL-1b, IL-6 and TNF-alpha cytokines. In the CFA and CCI model, the animals with COPD showed an increase in the mechanical hyperalgesia and the levels of IL-1b, IL-6 and TNF-alpha were greater in plasma up to 24 hours.
CONCLUSION: Animals with COPD have higher levels of pro-inflammatory cytokines and reduced nociceptive thresholds, suggesting a relationship between COPD and increased nociception.
Keywords
Resumo
JUSTIFICATIVA E OBJETIVOS: A doença pulmonar obstrutiva crônica (DPOC) caracteriza-se pela obstrução do fluxo aéreo e, embora comprometa os pulmões, produz importantes consequências sistêmicas. O objetivo deste estudo foi analisar a relação entre citocinas pró-inflamatórias e limiar nociceptivo em ratos com DPOC induzida por elastase pancreática suína.
MÉTODOS: Cento e quarenta e quatro animais foram distribuídos aleatoriamente em três modelos diferentes: dor fásica nociceptiva no teste de retirada de cauda (TF), dor inflamatória no modelo de adjuvante completo de Freund (CFA) e dor neuropática no modelo de constrição do nervo ciático (CCI). Vinte e um dias após a instilação traqueal de elastase a DPOC foi estabelecida e o limiar nociceptivo foi avaliado em diferentes períodos.
RESULTADOS: Os animais com DPOC apresentaram menor tempo de latência de retirada e maiores níveis das citocinas IL-1b, IL-6 e TNF-alfa no TF. No modelo CFA e CCI, os animais com DPOC apresentaram aumento da hiperalgesia mecânica e os níveis de IL-1b, IL-6 e TNF-alfa foram maiores no plasma até 24 horas.
CONCLUSÃO: Animais com DPOC apresentam níveis mais elevados de citocinas pró-inflamatórias e limiares nociceptivos reduzidos, sugerindo uma relação entre DPOC e aumento da nocicepção.
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References
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Submitted date:
12/20/2022
Accepted date:
02/24/2023
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